Beilstein J. Org. Chem.2016,12, 271–277, doi:10.3762/bjoc.12.29
formate dehydrogenase (FDH) preparations which can be used as effective biocatalysts along with functional oxidoreductases, in which in situ regeneration of NADH is required. For this purpose, Candida methylica FDH was covalently immobilized onto Immobead150 support (FDHI150), Immobead150 support
modified with ethylenediamine and then activated with glutaraldehyde (FDHIGLU), and Immobead150 support functionalized with aldehyde groups (FDHIALD). The highest immobilization yield and activity yield were obtained as 90% and 132%, respectively when Immobead150 functionalized with aldehyde groups was
, FDHIGLU and FDHIALD offer feasible potentials for in situ regeneration of NADH.
Keywords: biocatalysis; Candida methylica; formate dehydrogenase; Immobead150; regeneration of NADH; stabilization; Introduction
Dehydrogenases are one of the most promising enzymes in biocatalysis since these enzymes have
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Graphical Abstract
Figure 1:
The immobilization scheme of FDH onto Immobead 150 and modified Immobead 150 supports.